Figure 6
From: Keel petal incision: a simple and efficient method for genetic crossing in Medicago truncatula
Confirmation of successful crosses by nodule phenotypes of the progeny. M. truncatula plants were grown on an aeroponic chamber, as described in Materials and methods. Five days after germination, plants were starved for nitrogen for five days and then inoculated with Sinorhizobium meliloti. Root nodules were examined 15 days post-inoculation. A, Wild-type R108. B, NF11217 mutant. C -H, NF11217 X wild-type R108-BC1F1. The R108 plant (A) has pink wild-type nodules while those of NF11217 are white and ineffective (B). Each plant from the BC1F1 progeny (C-H) has pink nodules indicative of a successful cross. Bars = 250 μm.