Figure 4
From: Keel petal incision: a simple and efficient method for genetic crossing in Medicago truncatula
Steps in genetic crossing using the keel petal incision method. A, Unopened optimal female flower bud. B, Flower bud showing the keel petal under the standard petal indicating the site of incision on the central line of the keel petal. C, Flower bud showing the internal floral organs including anthers and stigma underneath the keel petal after making an incision. D, Flower bud showing stigma and style after the removal of anthers. E, Stigma covered with mature pollen after artificial cross-pollination. F, Closed flower bud after deposition of pollen on stigma. Bars = 0.5 mm.