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Figure 3 | Plant Methods

Figure 3

From: Keel petal incision: a simple and efficient method for genetic crossing in Medicago truncatula

Figure 3

M. truncatula flower development and manipulation of pollen donor flowers. A-G, Flowers from different developmental stages were collected, starting from when flower buds are first visible (Day 0). Flowers at day 3 are optimal to use as the female parent, while those at day 5 are best as the male parent. H, Day 5 flower. I, Day 5 flower with standard petal removed. J, Flower as in I, with stigma column exposed, showing anthers. K, Day 6 flower, rotated approximately 140° from flower in F. L, Day 6 flower with standard petal removed. M, flower as in L, rotated approximately 110°. Bar = 0.5 mm in top panel; bars = 1 mm in lower panels.

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