Figure 4From: A novel approach to the generation of seamless constructs for plant transformationVector linearization and RE analysis of cloned gfp . A. pAUrumII in its circular (supercoiled) and LguI treated, linear state with the LguI-spacer removed next to pAUrumIII in its circular (supercoiled) and BaeI treated, linear state. The BaeI treated pAUrumIII also appears in a relaxed, circular state. The short BaeI-spacer is not visible. B. Restriction analysis for pAUrumII-gfp with NotI on plasmid DNA from 15 colonies. The correct construct is separated in two fragments of 2.6 kb and 1.5 kb, respectively. C. Restriction analysis for pAUrumIII-gfp with AvrII. The correct construct is separated in two fragments of 2.6 kb and 1.5 kb, respectively. The construct separated in a 2.6 kb and a 0.86 kb fragment is pAUrumIII. For all gels, L indicates the O’GeneRuler 1 kb DNA Ladder (Thermo Scientific).Back to article page