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Figure 3 | Plant Methods

Figure 3

From: A novel approach to the generation of seamless constructs for plant transformation

Figure 3

PCR primer sequences. The fragment to be PCR amplified (A–I) and the orientation, forward (for) or reverse (rev), are indicated in the primer name in front of the primer sequence. Sequence with a single, black underline represents the part of the primer that anneals to the template. Sequence in bold, italic represents the 15 nt for In-Fusion™ cloning and pairwise coloring (A–H primers) represents homology; the same colors are used in Figure 1, indicating that the fragments are assembled from these homologous sequences, thus forming the two plasmids. Sequence with double underline represents recognition sites for LguI (A-rev, C-for) or BaeI (G-for), whereas sequence with dashed, red underline represents the monocotyledon Kozak consensus sequence (I-for).

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