Figure 1
From: A novel approach to the generation of seamless constructs for plant transformation
Maps of vectors and insert. pAUrumII prepared from the fragments A–E, pAUrumIII from F–H and the In-Fusion™-ready gene for green fluorescent protein, gfpIFR (the I fragment) are mapped with selected RE cleaving sites and nucleotide sequences. Colors of the fragment separators of the two plasmid maps correspond to the indications of sequence homology in Figure 3. Small green arrows indicate randomly generated sequences for optimized primer annealing (not used here) and the sequences can be removed with NarI and MluI.