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Table 2 Reproducibility test of phosphorylation site identification. +: detected; -: not detected.

From: Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites

Protein

At5g52300.1 low-temperature-responsive 65 kD protein

jgi code 153417 putative protein

Mw [Da]

65971

56515

Experiment

1

2

1

2

Sequence coverage [%]

65.8

67.5

29. 1

28.0

Peptide 1

GAVTSWLGGKPKpSPR

+

KLESAApTVAER

+

Peptide 2

LGYTGENGGGQSEpSPVKDETPR

+

VAVAPPSRPGpSGK

+

Peptide 3

LPLSGGGpSGVKETQQGEEK

+

SGpSAKVAVAPSR

-

Peptide 4

EAHQEPLNpTPVSLLSATEDVTR

+

  

Peptide 5

ESDINKNpSPARFGGESK

-

  

Peptide 6

MKVTDEpSPDQKSR

-