Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites

Table 2 Reproducibility test of phosphorylation site identification. +: detected; -: not detected.

Protein	At5g52300.1 low-temperature-responsive 65 kD protein		jgi code 153417 putative protein
Mw [Da]	65971		56515
Experiment	1	2	1	2
Sequence coverage [%]	65.8	67.5	29. 1	28.0
Peptide 1	GAVTSWLGGKPKpSPR	+	KLESAApTVAER	+
Peptide 2	LGYTGENGGGQSEpSPVKDETPR	+	VAVAPPSRPGpSGK	+
Peptide 3	LPLSGGGpSGVKETQQGEEK	+	SGpSAKVAVAPSR	-
Peptide 4	EAHQEPLNpTPVSLLSATEDVTR	+
Peptide 5	ESDINKNpSPARFGGESK	-
Peptide 6	MKVTDEpSPDQKSR	-

ISSN: 1746-4811