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Table 2 Reproducibility test of phosphorylation site identification. +: detected; -: not detected.

From: Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites

Protein At5g52300.1 low-temperature-responsive 65 kD protein jgi code 153417 putative protein
Mw [Da] 65971 56515
Experiment 1 2 1 2
Sequence coverage [%] 65.8 67.5 29. 1 28.0
Peptide 1 GAVTSWLGGKPKpSPR + KLESAApTVAER +
Peptide 2 LGYTGENGGGQSEpSPVKDETPR + VAVAPPSRPGpSGK +
Peptide 3 LPLSGGGpSGVKETQQGEEK + SGpSAKVAVAPSR -
Peptide 4 EAHQEPLNpTPVSLLSATEDVTR +   
Peptide 5 ESDINKNpSPARFGGESK -   
Peptide 6 MKVTDEpSPDQKSR -