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Table 1 Identified phosphorylation sites in seed proteins. Gene expression data were derived from a previous study [38].

From: Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites

Accession nr.

Description

Protein sequence coverage

Site of phosphorylation (designated as "p")

Seed specific expression

At5g52300.1

low-temperature-responsive 65 kD protein (LTI65)/desiccation-responsive protein 29B (RD29B)

65.8% (Mr 65971 Da)

MKVTDEpSPDQKSR

ESDINKNpSPARFGGESK

LPLSGGGpSGVKETQQGEEK

LGYTGENGGGQSEpSPVKDETPR

GAVTSWLGGKPKpSPR

EAHQEPLNpTPVSLLSATEDVTR

+

At3g12960.1

expressed protein similar to seed maturation protein from [Glycine max]

93% (Mr 9464 Da)

DIKDIKGTRTDDpSPR.-

+

At1g01100.1

60S acidic ribosomal protein P1 (RPP1A)

81.2% (Mr 11162 Da)

KKDEPAEEpSDGDLGFGLFD.-

-

At2g27710.1

60S acidic ribosomal protein P2 (RPP2B)

87% (Mr 11444 Da)

KEEKEEpSDDDMGFSLFE.-

-

At5g40420.1

glycine-rich protein/oleosin

42.2% (Mr 21279 Da)

HFQFQpSPYEGGR

+

At1g07985.1

Expressed protein

46.5% (Mr 16461 Da)

KLVDKVVGSSSPTNIHpSK

 

At5g50600.1

short-chain dehydrogenase/reductase (SDR) family protein similar to sterol-binding dehydrogenase steroleosin from [Sesamum indicum]

61% (Mr 39087 Da)

STLYPESIRTPEIKpSD.-

ELGpSPNVVTVHADVSKPDDCRR

+

At1g29350.1

expressed protein

14.6% (Mr 90879 Da)

SGpSTHFSSTDSGNFQGK

No data

At4g25580.1

stress-responsive protein-related contains weak similarity to Low-temperature-induced 65 kDa protein

57.8 % (Mr 66520 Da)

RGAPTLTPHNTPVSLLpSATEDVTR

GAPTLpTPHNTPVSLLSATEDVTR

+