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Figure 5 | Plant Methods

Figure 5

From: A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants

Figure 5

The automated ISH protocol on Arabidopsis tissues. Developing flowers (A-C), developing siliques (D), ovules (E) and transverse sections of the shoot apical meristem (SAM) of 10-day seedlings (F-G). Probes used are for histone H4 (A, D, E, G), AP3 (B), AG (C) and stm (F). (A-C) were counterstained with the cell wall dye, Calcofluor, which produces a light blue colour. Expression of AP3 and AG in serial sections (B and C) shows the distinct patterns of expression in the petal/stamen primordia for the class B AP3 gene and in the carpel primordia for the class C AG. In (D) arrows indicate expression of histone H4 in the developing ovules but by this stage there is no expression in the silique/carpel wall. Expression is also detectable in the endosperm of the developing ovule as well as in the cotyledons and root meristem of the embryo (E). In (F) an arrow indicates absence of stm expression in the leaf promordia but histone is expressed here and in the slightly older leaves (G). IM, inflorescence meristem; FM, floral meristem, number indicates the approximate flower stage; s, sepal; ca, carpel; st, stamen; pe, petal; cw, carpel wall; ov, ovule; en, endosperm; em, embryo; sam, shoot apical meristem; lp, leaf primordium.

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