Figure 4

Examples of gene expression patterns in developing wheat grains. ID numbers indicate the Incyte gene code and can be used to search the wheat in situ database at SCRI http://bioinf.scri.sari.ac.uk/cgi-bin/insitu/home (user: guest; password: wheatinsitu). (A) transcript detection specifically in the nucellar epidermis at 3DAA (ID 702007486). Arrowheads indicate no expression in the innermost nucellar lysate or the coenocytic endosperm. (B) coenocytic endosperm at 3DAA (ID 702038349) (C) peripheral cells of the modified aleurone only show transcript accumulation of an unknown gene at 9DAA (ID 701965703) (D) gene expression of a plantacyanin orthologue throughout the modified aleurone at 9DAA (ID 702044644). This section is from the same grain as in (C) and the arrowhead indicates that the most peripheral cells showing signal in (C) are not expressing plantacyanin. (E) a proteinase inhibitor is expressed strongly in the outer layers of the central endosperm at 9DAA (ID 701965839) (F) in contrast to (E), a gliadin storage protein gene is expressed throughout the central endosperm but not in the outermost layers (indicated with arrowhead; ID 702007003)