Figure 4
From: Cell type-specific characterization of nuclear DNA contents within complex tissues and organs
Confocal and biparametric flow cytometric analyses of wild-type and transgenic plants expressing nuclear GFP. Flow cytometry was done using a Cytomation MoFlo flow cytometer with laser excitation at 365/488 nm, and biparametric detection of DAPI fluorescence (418–482 nm; FL4; log units), and GFP fluorescence (505–530 nm; FL1; log units), with triggering based on 90°-light scatter [59]. For confocal microscopy, roots were counterstained by dipping in propidium iodide (1 μg/mL in water) for 2 minutes. Abbreviations: p35S: CaMV 35S promoter; pSCR: SCARECROW promoter; pSHR: SHORTROOT promoter; pRPL16B: ribosomal protein large subunit 16B promoter; pSultr2-1: sulfate transporter 2-1 promoter.