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Figure 4 | Plant Methods

Figure 4

From: Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli

Figure 4

gap repair HR rescue of the AtSTM locus. Schematic representation of the HR rescue of the AtSTM locus into the pBluescript gap repair vector (A) and the resultant pBlueAtSTM17 construct as expected from the correct recombination event. Diagnostic Eco RV sites are shown in blue, Pme I site in red, together with their co-ordinates within pBlueAtSTM17. Sal I and Hind III sites originally engineered in the homology arms of the gap repair vector are shown in green. (B). The results of Eco RV and Pme I restriction digestion of three plasmids resulting from independent recombination events from a single experiment, showing that only the plasmid in lane 3 resulted from the correct recombination. M1 = 1 kb ladder (New England Biolabs); M2 = High Molecular Weight Marker (Invitrogen).

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Plant Methods

ISSN: 1746-4811