Table 4 Composition of double-strength culture media. All concentrations are shown double-strength except for the supplements added after pH adjustment and sterilisation which are shown at their final concentrations.

MS Macro salts (×10)

200 ml

200 ml

200 ml

200 ml

200 ml

L7 Micro salts (×1000)

2 ml

2 ml

2 ml

2 ml

2 ml

FeNaEDTA (×100)

20 ml

20 ml

20 ml

20 ml

20 ml

MS vitamins (×1000)

2 ml

2 ml

-

-

-

Vitamins/Inositol (×200)

-

-

10 ml

10 ml

10 ml

Inositol

200 mg

200 mg

200 mg

200 mg

200 mg

Glutamine

1 g

1 g

-

-

-

Casein hydrolysate

200 mg

200 mg

-

-

-

MES

3.9 g

3.9 g

-

-

-

Glucose

20 g

-

-

-

-

Maltose

80 g

80 g

60 g

60 g

60 g

pH adjusted to 5.8 then autoclaved

pH adjusted to 5.7 then filter sterilised

2,4-D

2 mg

0.5 mg

0.1 mg

-

-

Picloram

2.0 mg

2.0 mg

-

-

-

Acetosyringone

200 μM

-

-

-

-

Timentin

-

160 mg

160 mg

160 mg

160 mg

Zeatin

-

-

5 mg

-

-

PPT

-

-

-

2–4 mg

3–4 mg