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Table 4 Composition of double-strength culture media. All concentrations are shown double-strength except for the supplements added after pH adjustment and sterilisation which are shown at their final concentrations.

From: Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Component Inoculation (/L) Induction (/L) RDZ (/L) RPPT (/L) R (/L)
MS Macro salts (×10) 200 ml 200 ml 200 ml 200 ml 200 ml
L7 Micro salts (×1000) 2 ml 2 ml 2 ml 2 ml 2 ml
FeNaEDTA (×100) 20 ml 20 ml 20 ml 20 ml 20 ml
MS vitamins (×1000) 2 ml 2 ml - - -
Vitamins/Inositol (×200) - - 10 ml 10 ml 10 ml
Inositol 200 mg 200 mg 200 mg 200 mg 200 mg
Glutamine 1 g 1 g - - -
Casein hydrolysate 200 mg 200 mg - - -
MES 3.9 g 3.9 g - - -
Glucose 20 g - - - -
Maltose 80 g 80 g 60 g 60 g 60 g
  pH adjusted to 5.8 then autoclaved pH adjusted to 5.7 then filter sterilised
2,4-D 2 mg 0.5 mg 0.1 mg - -
Picloram 2.0 mg 2.0 mg - - -
Acetosyringone 200 μM - - - -
Timentin - 160 mg 160 mg 160 mg 160 mg
Zeatin - - 5 mg - -
PPT - - - 2–4 mg 3–4 mg