Table 3 Composition of medium MG/L
From: Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat
Component | /litre |
|---|---|
Mannitol | 5 g |
L-Glutamic acid | 1 g |
KH2PO4 | 250 mg |
NaCl | 100 mg |
MgSO4·7H2O | 100 mg |
Tryptone | 5 g |
Yeast extract | 2.5 g |
pH 7.0 | Â |
Biotin (added after autoclaving from stock at 1 mg/100 ml (add 100 μl to 1 litre MG/L) | 1 μg |