Skip to main content

Advertisement

Table 2 Summary of Agrobacterium strains and vectors used to investigate wheat transformation.

From: Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Agrobacteriu m strain (binary vector) Chromosomal background Ti plasmid Opine classification Additional vir genes on binary or helper plasmids Binary type Selectable and scorable marker on T-DNA. (Promoter shown in parentheses)
ABI (pMON18365) [17, 18] C58 Disarmed pTiC58 Nopaline pMON18365, none reported normal-binary npt II (E35S) GUS (E35S)
C58C1 (pPTN155) [15] C58 Cured/disarmed? Nopaline pPTN155, none reported normal-binary npt II (35S) GUS (E35S)
AGL1 (pAL154/156) [21] C58, RecA pTiBo542 ΔT-DNA Succinamopine pAL154, 15.2 Kb fragment from pTiBo542 [47], pAL156, none super-binary bar (Ubi1) GUS (Ubi1)
AGL0 (pBGX1 and pTO134) [19] C58 pTiBo542 ΔT-DNA Succinamopine pBGX1, none reported pTO134, none reported normal-binary hpt (35S) gfp (Ubi1) bar (35S) sgfp S65T (35S)
AGL1 (pDM805) C58, RecA pTiBo542 ΔT Succinamopine pDM805, none reported normal-binary bar (Ubi1) GUS (Act1)
LBA4404 (pTOK233) [45] Ach5 DNA Disarmed pAL4404 Octopine pTOK233, extra copy of vir B, vir C and vir G from pTiBo542 47, [48] super-binary hpt (35S) GUS (35S)
LBA4404 (pHK21) [20] RecA Ach5 Disarmed pAL4404 Octopine pHK21, extra copy of vir B, vir C and vir G from pTiBo542 [47] super-binary bar (Ubi1) GUS (Ubi1)
AGL1 (pUNN-2) [46] C58, RecA pTiBo542 ΔT-DNA Succinamopine pUNN-2, none reported normal-binary npt II (Ubi1))
ABI (pMON30139 and others) [16] C58 Disarmed pTiC58 Nopaline pMON30139, none reported normal-binary aro A:CP4 (Act1) aro A:CP4 (e35S+ hsp intron)