Table 1 Summary of main parameters reported for Agrobacterium-mediated transformation of wheat.
From: Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat
Wheat variety (S – spring) (W – winter) | Explant type | Embryo Axis removed | Agrobacterium strain (binary vector) | Inoculation (Co-culture) *rt – room temp | Control of Agrobacterium cells | Plant selective agent | Transformation Freq. (%) | No of plants reported | Refs |
|---|---|---|---|---|---|---|---|---|---|
Bobwhite (S) | IE (age NS*); 1–6 d PCIE; 10–25 d EC | NS* | C58-ABI (pMON18365) | 3 h, 23–25°C (2–3 d, 24–26°C) | Carbenicillin (250 mg/l) | G418 | 1.4–4.3 | >100 | [17] |
Bobwhite (S) | 4 d PCIE | NS* | C58-ABI (pMON30139 and others) | 15–30 min, 23–25°C (2–3 d, 23–25°C) | Carbenicillin (250–500 mg/l) | Glyphosate | 4.4 | 3354 | [16] |
Bobwhite (S) | 1–6 d PCIE; 8–30 d EC | NS* | C58-ABI (pMON18365) | 5–60 min, 23–26°C (2–3 d, 24–26°C) | Carbenicillin (250 mg/l) | G418 Paromomycin Glyphosate | 4.8–19 | 154 | [18] |
Bobwhite (S) | 3–6 PCIE | NS* | C58C1 (pPTN155) | 45 min – 3 h, 25°C (1–3 d, 25°C) | Ticarcillium; Vancomycin Cefatoxin; (50 mg/l) | G418 | 0.5–1.5 | 13 | [15] |
Cadenza (S) Florida (W) | 0–72 h IE | Yes | AGL1 (pAL154/156) | 15 min-5 h, rt* (1–5 d, 24–25°C rt*) | Timentin (160 mg/l) | PPT (L-Phosphinothricin) | 0.3–3.3 | 44 | [21] |
Fielder (S) | 6–9 d PCIE | Yes | AGL0 (pBGX1) | 30–60 min rt* (2–3 d, 23–24°C) | Timentin (150 mg/l) | GFP, Bialaphos | 1.8 | 4 | [19] |
Veery-5 (S) | 14 d EC | Yes | LBA4404 (pHK21) | 15 min at rt* (1 d 27°C, 2 d 22°C) | Timentin (150 mg/l) | Glufosinate ammonium | 1.2–3.9 | 17 | [20] |
Vesna (S) | IE (age NS*) | NS* | LBA4404 (pTOK233) AGL1 (pDM805) | 15–30 min, (3 d, 27°C) | Cefotaxime (300 mg/l) | PPT (L-Phosphinothricin) | 0.13–0.41 | 6 | [45] |
Various Chinese varieties (NS*) | EC (age NS*) | NS* | AGL1 (pUNN-2) | 30–60 min (2 d, 28°C) | Timentin (150 mg/l) | Paromomycin | 3.7–5.9 | 44 | [46] |