Table 2 List of primers and T-DNA lines used
Species/gene | T-DNA knockout | Primer No | Primer (5'-3') |
|---|---|---|---|
AtSOC1 | GGATCGAGTCAGCACCAAACC CTTGAAGAACAAGGTAACCCA | ||
AtAGC1-10 | CGTTTCACTATCTCCTCCACAAG GGTGCTTTCAGAATGTTTACTAACGT | ||
AtWRKY22 | SALK_098205 | 2003 2004 | AAGAAAGTGTGCCATGTAGCAG CCGGAGACGATGAATAAGTAGC |
AtWRKY46 | GABI-Kat 038C07 | 182 712 | ATGGAGGAGGTTCTAGCGAGAGTC AAACGTCTTTACCATCATCAAGC |
AtWRKY53 | SALK_034157 | 713 714 | ACGAATTGGAACTAGGGAAAGAG CCATCATCAATAGAGCCATTTTC |
AtWRKY36 | GABI-Kat 258B10 | 72 136 | CCTGCCTACAAAGATCATCTAGTTTCG ATGATCAAAGAGGAGACCGTTTC |
AtWRKY70 | GABI-Kat 752F08 | ATGGATACTAATAAAGCAAAAAAGC AGATAGATTCGAACATGAACTGAAG | |
AtWRKY18 | GABI-Kat 328G03 | CATGGGTTCATTTCAAATTTTCG CGATCTGCTCATGTTGCTGATGATG | |
NtCDPK2 | ATGGGCAACGCATGCGGCGG GATGACTCTCAAAGCCATTTTC | ||
NtRBCS | CCTCTGCAGCAGTTGCCACC CCTGTGGGTATGCCTTCTTC | ||
Hordeum vulgare | 28-12A | ATACCTGCACAGCCACAAGTC GCAACTTCGCCTCTACGTTC | |
Hordeum vulgare | 29-19A | ACATGTGAGCTTGCTGGTTG TGGGGGATGGTTAATGGTAG | |
Hordeum vulgare | 33-22A | CCTGCCGATGTAATCTGGTT GATCTTTGCCATGTCTGTTTCG | |
Hordeum vulgare | 41-30A | AACATGCAAGCACACGTCAT CATGATTGCTGTGGCTGACT |