Table 2 List of primers and T-DNA lines used
| Species/gene | T-DNA knockout | Primer No | Primer (5'-3') |
|---|---|---|---|
| AtSOC1 |
GGATCGAGTCAGCACCAAACC CTTGAAGAACAAGGTAACCCA | ||
| AtAGC1-10 |
CGTTTCACTATCTCCTCCACAAG GGTGCTTTCAGAATGTTTACTAACGT | ||
| AtWRKY22 | SALK_098205 |
2003 2004 |
AAGAAAGTGTGCCATGTAGCAG CCGGAGACGATGAATAAGTAGC |
| AtWRKY46 | GABI-Kat 038C07 |
182 712 |
ATGGAGGAGGTTCTAGCGAGAGTC AAACGTCTTTACCATCATCAAGC |
| AtWRKY53 | SALK_034157 |
713 714 |
ACGAATTGGAACTAGGGAAAGAG CCATCATCAATAGAGCCATTTTC |
| AtWRKY36 | GABI-Kat 258B10 |
72 136 |
CCTGCCTACAAAGATCATCTAGTTTCG ATGATCAAAGAGGAGACCGTTTC |
| AtWRKY70 | GABI-Kat 752F08 |
ATGGATACTAATAAAGCAAAAAAGC AGATAGATTCGAACATGAACTGAAG | |
| AtWRKY18 | GABI-Kat 328G03 |
CATGGGTTCATTTCAAATTTTCG CGATCTGCTCATGTTGCTGATGATG | |
| NtCDPK2 |
ATGGGCAACGCATGCGGCGG GATGACTCTCAAAGCCATTTTC | ||
| NtRBCS |
CCTCTGCAGCAGTTGCCACC CCTGTGGGTATGCCTTCTTC | ||
| Hordeum vulgare | 28-12A |
ATACCTGCACAGCCACAAGTC GCAACTTCGCCTCTACGTTC | |
| Hordeum vulgare | 29-19A |
ACATGTGAGCTTGCTGGTTG TGGGGGATGGTTAATGGTAG | |
| Hordeum vulgare | 33-22A |
CCTGCCGATGTAATCTGGTT GATCTTTGCCATGTCTGTTTCG | |
| Hordeum vulgare | 41-30A |
AACATGCAAGCACACGTCAT CATGATTGCTGTGGCTGACT |