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Figure 3 | Plant Methods

Figure 3

From: A rapid and versatile combined DNA/RNA extraction protocol and its application to the analysis of a novel DNA marker set polymorphic between Arabidopsis thaliana ecotypes Col-0 and Landsberg erecta

Figure 3

Use of the Sucrose Prep to identify T-DNA insertion knock-out mutations. Putative mutant plants homozygous for the T-DNA insertion were identified with the Sucrose Prep using gene specific primers for AtWRKY22 (Table 2) and amplification of DNA by PCR. The lines were also tested by RT-PCR for the loss of AtWRKY22 transcript using a commercial RNA isolation kit (Qiagen). Positions of the primers used for amplification are indicated below the schematic diagram.

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