Chromosomal location of polymorphic markers and their use for genotyping. (A) Chromosomal location of the 51 polymorphic Col-0/Ler markers is depicted graphically and the sequence of each primer pair and fragment polymorphism is listed in Table 1. The region on chromosome II that is linked to the early flowering phenotype is boxed. (B) PCR amplification of DNA markers F14G16 on chromosome IV and F26B6 on chromosome II from the parents and early and normal flowering F2 DNA bulks. For DNA marker F14G16 both the Ler and Col-0 alleles were amplified from the early flowering bulk demonstrating that the marker is not linked to the early flowering mutation. Only the Ler allele of DNA marker F26B6 was amplified from the early flowering DNA bulk indicating the marker is linked to the early flowering mutation. (C) PCR amplification of DNA marker F26B6 from the parents and progeny.