Efficiency of PCR amplification using DNA templates prepared by the Sucrose Prep. (A) Extracts were prepared from various tissues using 10 mg sample/200 μl Sucrose Solution. (1) 4 week-old leaf, (2) senescent leaf, (3) anthers, (4) petals, (5) sepals, (6) gynoecium, (7) petiole, (8) 8–12 days old embryos. (B) Comparison of DNA templates prepared by the methods of Edwards (ED; Edwards et al., ) and Sucrose Prep (SP) in long-range PCR amplification. Optimized PCR conditions were used to amplify 2.2, 3.7, and 4 kb size products. (C) Long-range PCR amplification on DNA template isolated by CTAB (CT; see methods) and Sucrose Prep using LA TaKaRa polymerase. Sucrose Prep replicates show the inhibition of PCR amplification by inclusion of plant debris in the reaction (SP1) in comparison to samples avoiding contamination with debris (SP2).