A procedure for localisation and electrophysiological characterisation of ion channels heterologously expressed in a plant context

Table 1 A comparison of the values of voltage-gating parameters of KAT1 and AKT1 channels in different expression contexts (see text).

Channel		Mean value for voltage-gating parameters of the channel in						References
		Animal cells			Yeast cells	Plant cells
		Xenopus oocytes	insect cells ( Sf 9)	Green monkey cos cells		Tobacco mesophyll cells	Arabidopsis native cells
AKT1	Activation potential (mV)	No current⁽¹⁾	-70/-80 ⁽¹⁾	No current⁽²⁾	-140⁽³⁾	-40	-40⁽⁴⁾	⁽¹⁾ Ref [16]
	Gating charge	No current⁽¹⁾	1.5⁽¹⁾	No current⁽²⁾	n.r.^(3),*	1.3 ± 0.2 (n = 5)	n.r. ^(4),*	⁽²⁾ Erwan Michard, unpublished ⁽³⁾ Ref. [34]
	Half-activation potential (mV)	No current⁽¹⁾	-123⁽¹⁾	No current⁽²⁾	n.r.^(3),*	-95 ± 10 (n = 5)	n.r. ^(4),*	⁽⁴⁾ Ref. [40] ⁽⁵⁾ Ref. [35]
KAT1	Activation potential (mV)	-80⁽⁵⁾	- 60/-80⁽⁶⁾	- 100⁽⁷⁾	- 120⁽³⁾	-120	-120 ⁽⁸⁾	⁽⁶⁾ Ref. [38] ⁽⁷⁾ Ref. [37]
	Gating charge	1.7 ± 0.2 (n = 5)⁽⁹⁾	n.r.^(6),*	1.6⁽⁷⁾	n.r.^(3),*	1.7 ± 0.1 (n = 4)	1.6 ± 0.2 (n = 5)⁽¹⁰⁾	⁽⁸⁾ Ref. [46] ⁽⁹⁾ Ref. [36]
	Half-activation potential (mV)	-138 ± 7 (n = 5)⁽⁹⁾	n.r. ^(6),*	-135⁽⁷⁾	n.r. ^(3),*	-164 ± 6 (n = 4)	-157 ± 7 (n = 5)⁽¹⁰⁾	⁽¹⁰⁾ Eric Hosy, unpublished * not reported by these authors

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