Figure 2
From: Blue-native PAGE in plants: a tool in analysis of protein-protein interactions
Second and third dimensions for BN-PAGE of Arabidopsis mitochondrial protein complexes. A) BN-PAGE of a digitonin-solubilized fraction followed by denaturing and reducing SDS-PAGE (BN/SDS-PAGE); B) BN-PAGE of Digitonin-solubilized protein complexes followed by a second BN-PAGE (BN/BN-PAGE) employing n-Dodecylmaltoside to introduce slightly less native conditions inducing partial dissociation of protein supercomplexes; C) IEF/SDS-PAGE of the ATP-Synthase band of a dodecylmaltoside-solubilized sample. The band has been cut out of the gel and the proteins have been subsequently electroeluted under denaturing conditions followed by a precipitation step to concentrate and purify the proteins for isoelectric focussing. The image (c) was reproduced from figure 1 in [15] with permission from Wiley-VCH.