Mutational optimization of the coelenterazine-dependent luciferase from Renilla
© Woo and von Arnim; licensee BioMed Central Ltd. 2008
Received: 09 August 2008
Accepted: 30 September 2008
Published: 30 September 2008
Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated. We titrated that one RLUC molecule can turn over about one hundred molecules of coelenterazine substrate. Mutagenesis of active site residue Pro220 extended the half-life of photon emission, yielding brighter luminescence in E. coli. Random mutagenesis uncovered two new mutations that stabilized and increased photon emission in vivo and in vitro, while ameliorating substrate inhibition. Further amended with a previously identified mutation, a new triple mutant showed a threefold improved kcat, as well as elevated luminescence in Arabidopsis. This advances the utility of RLUC as a reporter protein, biosensor, or resonance energy donor.
Renilla luciferase (RLUC) is a cofactor-less, single subunit, blue light emitting luciferase isolated from the marine anthozoan Renilla reniformis (RLUC, E.C. number 126.96.36.199, luciferin-2-monooxygenase, decarboxylating) . Aside from its utility as a reporter for gene expression assays, RLUC has also found application in assays for protein interaction based on fragment complementation  and bioluminescence resonance energy transfer . The substrate of Renilla luciferase, coelenterazine, consists of a central aromatic imidazopyrazinone, which is derivatized with p-hydroxy-phenyl (R1), benzyl (R2), and p-hydroxy-benzyl (R3) moieties. Using molecular oxygen, RLUC catalyzes an oxidative decarboxylation in which the imidazole ring is opened and carbon dioxide is released [4–7]. Relaxation of the electronically excited coelenteramide reaction product is accompanied by emission of a photon of blue (~470 nm) light.
Compared to the calcium-stimulated photoprotein, aequorin, and its relatives, which utilize the same substrate as RLUC, the catalytic mechanism of RLUC is not yet well understood [8, 9]. Photoproteins are single turnover enzymes. Removal of the coelenteramide product and binding of a fresh substrate molecule require a reducing agent and the concomitant removal of calcium . RLUC is not homologous to aequorin but evolved from an α/β hydrolase ancestor closely related to current bacterial dehalogenases. Its structure has recently been solved . Within the large hydrophobic active site, the putative catalytic triad consists of Asp120, His285, and Glu144. Mutagenesis data and inactivation with diethylpyrocarbamate indicate that His285 is important for catalysis, presumably as a general base [12, 13]. A model for how coelenterazine and its peroxidized reaction intermediate might be positioned in the active site has been proposed .
Re-engineering of the RLUC sequence might ameliorate undesirable properties that arise upon expression in heterologous hosts, which lack RLUCs two partner proteins, a green fluorescent protein and a calcium-responsive coelenterazine binding protein [14, 15]. Previous consensus-guided mutagenesis has already led to RLUC versions with improved stability in serum, improved ability to utilize the purple-emitting substrate, bisdeoxycoelenterazine, and altered spectral properties [12, 16]. RLUC is well known to be inactivated in the presence of substrate, resulting in most of the light to be emitted as a flash of a few seconds in length. While a short half-life of the enzyme might be beneficial for time-resolved gene expression studies, it is undesirable for protein-interaction studies based on bioluminescence resonance energy transfer [5, 17, 18].
Here, we describe the results of site directed and random mutagenesis in conjunction with expression and purification of recombinant RLUC enzyme in E. coli with the goal of improving specific enzymatic parameters of RLUC. We describe novel mutants with increased kcat, extended half-life of photon emission in vitro and in vivo, and enhanced light emission upon expression in plant cells.
Mutagenesis and other recombinant DNA techniques
The wild type Renilla reniformis luciferase cDNA obtained from plasmid pBS-35S-RLUC-attR (Genbank accession, AY995136)  was subcloned into the expression vector pET30(a) as an Nco I-Bam HI fragment, thus adding an N-terminal histidine tag and linker sequence (His-RLUC) . For random mutagenesis, the RLUC cDNA was amplified using an error-prone PCR procedure, GeneMorpho®II Random Mutagenesis (Stratagene, La Jolla, CA). A library of 1300 putative mutant clones (strain BL21(DE3)) was grown in LB in white 96-well microtiter plates (Packard, Meriden, CT) to an optical density of about 0.6. Colonies were surveyed for RLUC activity in the presence of 2 μM native coelenterazine (Biotium, Hayward, CA) in the PolarStar plate reader (BMG Labtech, Durham, NC). Candidates with elevated RLUC activity were reconfirmed by inducing RLUC expression at OD = 0.5 with 1 mM IPTG for 1 hour at 30°C and the mutation was identified by DNA sequencing. Subsequently, mutations were also introduced into a human codon-optimized RLUC cDNA (hRLUC, Genbank accession, AAK53368, Packard, Meriden, CT). Site directed mutagenesis was performed using the Quickchange procedure (Stratagene, La Jolla, CA). Mutations were confirmed by resequencing of the entire RLUC coding region to guard against unintended secondary mutations. To generate recombination cloning vectors, the appropriate fragments of pBS-35S-hRLUC-attR (Genbank accession AY995138) and pBS-35S-attR-hRLUC (Genbank accession AY995140)  were replaced with the corresponding NheI/BglII restriction fragment from pET30(a)-SuperhRLUC that contained the M185V, K189V, and V267I mutations. The entire SuperhRLUC coding regions of the recombination vectors were confirmed by sequencing.
Expression and purification of RLUC
RLUC expression in E. coli strain BL21(DE3)pLysS was induced with 1 mM IPTG for 3 h hours at 30°C. The accumulation of RLUC in E. coli was routinely checked by cell lysis and gel electrophoresis and Coomassie Blue-staining. Mutant proteins generally accumulated to similar levels. RLUC was purified from the soluble cytosolic fraction over a nickel column (His-Bind Kit, Novagen, Darmstadt, Germany) following standard procedures that included sonication, centrifugation of cell debris at 12,000 rpm for 10 minutes at 4°C, and filtration of the supernatant through a 0.45 micron filter to prevent clogging of resin. Protein was affinity purified according to the manufacturer's protocol and eluted with 1 M imidazole, 0.5 M NaCl, 20 mM Tris-HCl, pH, 7.9. After elution, RLUC was dialyzed overnight against 2 L of phosphate buffered saline (PBS, pH7.2) in order to remove imidazole. Protein concentration was determined using the BCA assay (Pierce, Rockford, IL) with BSA as a standard. Alternatively, the protein concentration of preparations that were free of imidazole was measured by UV-absorbance using an extinction coefficient of 65,040 M-1 cm-1 . Purified RLUC protein was stored in PBS with 50% glycerol at -70°C in small aliquots or stored at 4°C for up to 2 weeks.
Kinetics of RLUC enzyme activity
Enzyme assays were conducted using freshly purified RLUC enzyme at a concentration of 1 nM or as otherwise indicated in 1 ml PBS (pH 7.2). Native coelenterazine substrate was added from a 250× stock solution of the indicated concentration in ethanol (final ethanol concentration, 0.8%), the solution was mixed by tapping to ensure a maximal supply of oxygen, and the luminescence activity was recorded in the TD20/20 luminometer (Turnerdesigns, Sunnyvale, CA). The first 5-second luminescence reading was taken as a measure of enzyme activity. The KM values of wild type RLUC and selected mutants were calculated according to standard Michaelis-Menten theory using Prism software (GraphPad Software Inc., San Diego, CA) from at least 3 repeat measurements. Several independent protein preparations yielded similar KM values.
Luminescence spectra were recorded under the same condition as the enzyme assay using a spectroluminometer (Photon Technology International, Inc., Birmingham, NJ), except that the assay volume was 2 ml. Native coelenterazine substrate was 2 μM (ethanol concentration, 0.8%). Protein concentration was 10 nM purified enzyme or as otherwise indicated. Generally, the emission spectrum was analyzed with the Felix32 software (Photon Technology International, Inc., Birmingham, NJ). All spectra were recorded at 1 nm per second from short to long wavelength. No adjustments for detector sensitivity or luminescence decay over time were made; nevertheless, the spectra are directly comparable among each other and emphasize the differences in emission in the short-wavelength region.
The amount of RLUC enzyme needed to deplete a nearly saturating amount of substrate (0.1 ml of 1 μM or 10 μM coelenterazine) was determined by titration. Parallel reactions were set up with RLUC at concentrations between 100 nM and 1 nM in 100 μl of assay buffer (50 mM potassium phosphate, pH 7.4, 500 mM NaCl, 1 mM EDTA) . Reactions were allowed to proceed for at least 2 hours until luminescence had decayed to near-background levels. Each spent reaction was then split into two aliquots. To one aliquot fresh enzyme (10 nM) was added. If no increase in luminescence was observed it was concluded that the substrate must have been used up completely allowing us to deduce the stoichiometry between enzyme and substrate. The second 50 μl aliquot was supplemented with 10 μM substrate to check whether the substrate had been used up completely by the enzyme or whether RLUC activity had been depleted.
Spectrophotometric assay of RLUC activity
The spectrum of a 10 μM solution of coelenterazine in assay buffer was recorded. RLUC was added to 100 nM and the spectrum re-recorded after 10 minutes and 60 minutes of reaction time. The extent of spontaneous degradation of coelenterazine was determined in a control reaction without added enzyme.
Plant growth condition and transgenic lines
Columbia wild type and transgenic seedlings were germinated on 0.8% agar medium containing Murashige and Skoog salts (MS; Sigma, St. Louis) and 1% sucrose without antibiotics. The transgenic plants expressing the hRLUC or SuperhRLUC cassettes were grown on a MS selection media containing 1.5 mg/l ammonium glufosinate herbicide (Basta; Sigma, St. Louis).
Measurement of Renilla luciferase activity in transgenic Arabidopsis
Luminescence units were measured from 10 day-old seedlings in the presence of 2 μM coelenterazine (Biotium, Hayward, CA) in water using a TD-20/20 tube luminometer that is equipped with the blue-color filter. After adding 2 μM coelenterazine, samples were incubated in darkness for 10 min at room temperature to allow the substrate to penetrate into plants and to allow delayed chlorophyll autofluorescence to decay .
Protein extraction from plants and western blotting
Plant protein was extracted with passive lysis buffer from the Dual-Luciferase® Reporter Assay System (Promega, Madison, WI) and RLUC detected by western blotting using a monoclonal antibody (Chemicon, Temecula, CA).
Transgenic 7–10 days old Arabidopsis seedlings were imaged on a Nikon microscope with a Hamamatsu C2400 ICCD (Meyer Instruments, Houston, TX). Seedlings were pre-incubated in 2 μM coelenterazine for 5 min and then photon emission was recorded for 5 min. Images were pseudo-colored with ImageJ (NIH, Bethesda, MD).
Results and discussion
Activities of RLUC mutants selected for improved enzymatic activity.
Activity ± SD
Native RLUC cDNA
in vitro 1)
163 ± 33
128 ± 16
317 ± 82
411 ± 113
Codon-optimized RLUC cDNA (hRLUC)
in vivo 2)
175 ± 70
425 ± 120
475 ± 130
The coelenteramide reaction product relaxes from the excited state to the ground state under emission of a photon of light. The precise peak wavelength of light emission is influenced by the protonation state of the reaction product and is also dependent on the physical environment (hydrophobicity) in the active site . The emission spectra of K189V and M185G were similar to wild-type RLUC (not shown). In contrast, V267I showed two major peaks, a blue-shifted shoulder of 390 nm, indicative of the formation of a neutral coelenteramide [7, 21–23] and a blue-shifted maximum at 450 nm [see Additional file 1].
Mutant versions of Renilla luciferase with increased kcat were identified from a library of random mutations expressed in E. coli. A combination of two or three mutations resulted in increased activity of the Renilla luciferase reporter enzyme in transgenic Arabidopsis.
relative light units
This work was supported by National Science Foundation grants MCB-0114653 and DBI-0619631. We are grateful for enzymological advice from Liz Howell and Dan Roberts and comments on the manuscript by Byung-Hoon Kim. We thank Tim Sparer for access to the spectroluminometer and the Center for Environmental Biotechnology for access to the photon counting camera.
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